Arleen SorkinHarley Quinn / Dr. Harleen Quinzel (voice)
Diane PershingPoison Ivy / Dr. Pamela Isley (voice)
Tara StrongBatgirl / Barbara Gordon (voice)
Adrienne BarbeauCatwoman / Selina Kyle / Renée Montoya (voice)
Tom KennySam (voice)
Jennifer HaleDora Smithy / Selma Reesedale (voice)